• Immunofluorescence analysis of L929 cells using DRG2 Rabbit polyclonal antibody (STJ119583) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using DRG2 Rabbit polyclonal antibody (STJ119583) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using DRG2 Rabbit polyclonal antibody (STJ119583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DRG2 antibody (150-260) (STJ119583)

SKU:
STJ119583

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DRG2 (150-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DRG2
Gene ID: 1819
Uniprot ID: DRG2_HUMAN
Immunogen Region: 150-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-260 of human DRG2 (NP_001379.1).
Immunogen Sequence: TKGEVQRSLLEKELESVGIR LNKHKPNIYFKPKKGGGISF NSTVTLTQCSEKLVQLILHE YKIFNAEVLFREDCSPDEFI DVIVGNRVYMPCLYVYNKID QISMEEVDRLA
Tissue Specificity Highest levels in skeletal muscle, heart and kidney. Low levels in colon, thymus, spleen, small intestine, lung and Leukocytes.
Post Translational Modifications Hydroxylated (with S stereochemistry) at C-3 of Lys-21 by JMJD7.this modification hinders trypsin-catalyzed proteolysis in vitro. Polyubiquitinated.
Function Catalyzes the conversion of GTP to GDP through hydrolysis of the gamma-phosphate bond in GTP. When hydroxylated at C-3 of 'Lys-21' by JMJD7, may bind to RNA and play a role in translation.
Protein Name Developmentally-Regulated Gtp-Binding Protein 2
Drg-2
Translation Factor Gtpase Drg2
Trafac Gtpase Drg2
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Developmentally-Regulated Gtp-Binding Protein 2 antibody
Anti-Drg-2 antibody
Anti-Translation Factor Gtpase Drg2 antibody
Anti-Trafac Gtpase Drg2 antibody
Anti-DRG2 antibody

Information sourced from Uniprot.org

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