Anti-DRG1 antibody (158-367) (STJ23436)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DRG1 (158-367) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DRG1 |
| Gene ID | 4733 |
| Uniprot ID | DRG1_HUMAN |
| Immunogen Region | 158-367 |
| Immunogen Sequence | KKIIENELEGFGIRLNSKPP NIGFKKKDKGGINLTATCPQ SELDAETVKSILAEYKIHNA DVTLRSDATADDLIDVVEGN RVYIPCIYVLNKIDQISIEE LDIIYKVPHCVPISAHHRWN FDDLLEKIWDYLKLVRIYTK PKGQLPDYTSPVVLPYSRTT VEDFCMKIHKNLIKEFKYAL VWGLSVKHNPQKVGKDHTLE DEDVIQIVKK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 158-367 of human DRG1 (NP_004138.1). |
Additional Info
| Tissue Specificity | High levels in skeletal muscle, heart, and kidney. Intermediate levels in liver, placenta and brain. Low levels in colon, thymus, spleen, small intestine, lung and leukocytes. |
| Post Translational Modifications | Sumoylated by UBE2I in response to MEKK1-mediated stimuli. Phosphorylated at Thr-100 by STK16. Hydroxylated (with S stereochemistry) at C-3 of Lys-22 by JMJD7.this modification hinders trypsin-catalyzed proteolysis in vitro. Polyubiquitinated.this modification induces proteolytic degradation and is impaired by interaction with ZC3H15. |
| Function | Catalyzes the conversion of GTP to GDP through hydrolysis of the gamma-phosphate bond in GTP. Appears to have an intrinsic GTPase activity that is stimulated by ZC3H15/DFRP1 binding likely by increasing the affinity for the potassium ions. When hydroxylated at C-3 of 'Lys-22' by JMJD7, may bind to RNA and play a role in translation. Binds to microtubules and promotes microtubule polymerization and stability that are required for mitotic spindle assembly during prophase to anaphase transition. GTPase activity is not necessary for these microtubule-related functions. |
| Protein Name | Developmentally-Regulated Gtp-Binding Protein 1Drg-1Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 3Nedd-3Translation Factor Gtpase Drg1Trafac Gtpase Drg1 |
| Database Links | Reactome: R-HSA-9629569 |
| Cellular Localisation | NucleusCytoplasmThe Drg1-Zc3h15/Dfrp1 Complex Associates With Polysomes |
| Alternative Antibody Names | Anti-Developmentally-Regulated Gtp-Binding Protein 1 antibodyAnti-Drg-1 antibodyAnti-Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 3 antibodyAnti-Nedd-3 antibodyAnti-Translation Factor Gtpase Drg1 antibodyAnti-Trafac Gtpase Drg1 antibodyAnti-DRG1 antibodyAnti-NEDD3 antibody |
Information sourced from Uniprot.org