STJ71001 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-DRD5 antibody (Internal) (STJ71001)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71001-100
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General Information

Short DescriptionGoat polyclonal antibody anti-DRD5 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Preliminary testing showed a band at approx 55kDa in Caco-2 and U2OS cell lysate after 1-2µg/ml antibody staining (calculated MW of 53.0kDa according to NP_000789.1). Primary incubati
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolDRD5
Gene ID1816
Uniprot IDDRD5_HUMAN
Accession NumberNP_000789.1
Immunogen RegionInternal
Immunogen SequenceNREVDNDEEEGPFD

Additional Info

Function Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Protein Name D(1b Dopamine Receptor
D(5 Dopamine Receptor
D1beta Dopamine Receptor
Dopamine D5 Receptor
Database Links Reactome: R-HSA-390651
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-D(1b Dopamine Receptor antibody
Anti-D(5 Dopamine Receptor antibody
Anti-D1beta Dopamine Receptor antibody
Anti-Dopamine D5 Receptor antibody
Anti-DRD5 antibody
Anti-DRD1B antibody
Anti-DRD1L2 antibody

Information sourced from Uniprot.org

Citations

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