• Western blot analysis of various lysates using DRAP1 Rabbit polyclonal antibody (STJ119678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-DRAP1 antibody (50-150) (STJ119678)

SKU:
STJ119678

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DRAP1 (50-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DRAP1
Gene ID: 10589
Uniprot ID: NC2A_HUMAN
Immunogen Region: 50-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-150 of human DRAP1 (NP_006433.2).
Immunogen Sequence: LKKACQVTQSRNAKTMTTSH LKQCIELEQQFDFLKDLVAS VPDMQGDGEDNHMDGDKGAR RGRKPGSGGRKNGGMGTKSK DKKLSGTDSEQEDESEDTDT D
Tissue Specificity Ubiquitous. Highly expressed in adult testis, heart, skeletal muscle, pancreas and brain, and in fetal brain, liver and kidney.
Post Translational Modifications Phosphorylation reduces DNA binding, but has no effect on heterodimerization and TBP binding.
Function The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.
Protein Name Dr1-Associated Corepressor
Dr1-Associated Protein 1
Negative Cofactor 2-Alpha
Nc2-Alpha
Database Links Reactome: R-HSA-1181150
Reactome: R-HSA-1502540
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dr1-Associated Corepressor antibody
Anti-Dr1-Associated Protein 1 antibody
Anti-Negative Cofactor 2-Alpha antibody
Anti-Nc2-Alpha antibody
Anti-DRAP1 antibody

Information sourced from Uniprot.org

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