• Western blot analysis of various lysates using DPPA4 Rabbit polyclonal antibody (STJ115677) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DPPA4 antibody (15-304) (STJ115677)

SKU:
STJ115677

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DPPA4 (15-304) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DPPA4
Gene ID: 55211
Uniprot ID: DPPA4_HUMAN
Immunogen Region: 15-304
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 15-304 of human DPPA4 (NP_060659.3).
Immunogen Sequence: KGKEWTSTEKSREEDQQASN QPNSIALPGTSAKRTKEKMS IKGSKVLCPKKKAEHTDNPR PQKKIPIPPLPSKLPPVNLI HRDILRAWCQQLKLSSKGQK LDAYKRLCAFAYPNQKDFPS TAKEAKIRKSLQKKLKVEKG ETSLQSSETHPPEVALPPVG EPPALENSTALLEGVNTVVV TTSAPEALLASWARISARAR TPEAVESPQEASGVRWCVVH GKSLPADTDGWVHLQFHAG
Function May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
Protein Name Developmental Pluripotency-Associated Protein 4
Database Links Reactome: R-HSA-2892247
Cellular Localisation Nucleus
Associated With Transcriptionally Active Chromatin
Alternative Antibody Names Anti-Developmental Pluripotency-Associated Protein 4 antibody
Anti-DPPA4 antibody

Information sourced from Uniprot.org

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