Anti-DPPA3 antibody (STJA0009788)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human STELLAR
General Information
| Short Description | Rabbit polyclonal anti-Developmental pluripotency-associated protein 3 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DPPA3 |
| Gene ID | 359787 |
| Uniprot ID | DPPA3_HUMAN |
| Immunogen | Synthesized peptide derived from human STELLAR |
| Specificity | This antibody detects endogenous levels of STELLAR at Human, Mouse, Rat |
Additional Info
| Function | Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis. |
| Protein Name | Developmental Pluripotency-Associated Protein 3Stella-Related Protein |
| Database Links | Reactome: R-HSA-9821002 |
| Cellular Localisation | NucleusCytoplasmMainly Localizes In The Female PronucleusLocalization To The Male Pronucleus In Much Weaker |
| Alternative Antibody Names | Anti-Developmental Pluripotency-Associated Protein 3 antibodyAnti-Stella-Related Protein antibodyAnti-DPPA3 antibodyAnti-STELLAR antibody |
Information sourced from Uniprot.org