| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DPP8 (10-230) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DPP8 |
| Gene ID: |
54878 |
| Uniprot ID: |
DPP8_HUMAN |
| Immunogen Region: |
10-230 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 10-230 of human DPP8 (NP_932065.1). |
| Immunogen Sequence: |
IKSGKCNMAAAMETEQLGVE IFETADCEENIESQDRPKLE PFYVERYSWSQLKKLLADTR KYHGYMMAKAPHDFMFVKRN DPDGPHSDRIYYLAMSGENR ENTLFYSEIPKTINRAAVLM LSWKPLLDLFQATLDYGMYS REEELLRERKRIGTVGIASY DYHQGSGTFLFQAGSGIYHV KDGGPQGFTQQPLRPNLVET SCPNIRMDPKLCPADPDWIA F |
| Tissue Specificity | Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain. |
| Function | Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8. Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation. The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8.however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition. |
| Protein Name | Dipeptidyl Peptidase 8Dp8Dipeptidyl Peptidase Iv-Related Protein 1Dprp-1Dipeptidyl Peptidase ViiiDpp ViiiProlyl Dipeptidase Dpp8 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Dipeptidyl Peptidase 8 antibodyAnti-Dp8 antibodyAnti-Dipeptidyl Peptidase Iv-Related Protein 1 antibodyAnti-Dprp-1 antibodyAnti-Dipeptidyl Peptidase Viii antibodyAnti-Dpp Viii antibodyAnti-Prolyl Dipeptidase Dpp8 antibodyAnti-DPP8 antibodyAnti-DPRP1 antibodyAnti-MSTP097 antibodyAnti-MSTP135 antibodyAnti-MSTP141 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89103/357560/STJ11100031_1__14868.1713121685.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
