• Immunofluorescence analysis of U2OS cells using DPM1 antibody (STJ110068) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DPM1 antibody (STJ110068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DPM1 antibody (1-260) (STJ110068)

SKU:
STJ110068

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DPM1 (1-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DPM1
Gene ID: 8813
Uniprot ID: DPM1_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DPM1 (NP_003850.1).
Immunogen Sequence: MASLEVSRSPRRSRRELEVR SPRQNKYSVLLPTYNERENL PLIVWLLVKSFSESGINYEI IIIDDGSPDGTRDVAEQLEK IYGSDRILLRPREKKLGLGT AYIHGMKHATGNYIIIMDAD LSHHPKFIPEFIRKQKEGNF DIVSGTRYKGNGGVYGWDLK RKIISRGANFLTQILLRPGA SDLTGSFRLYRKEVLEKLIE KCVSKGYVFQMEMIVRARQL NYTIGEVPISFVDRVYGES
Function Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.catalytic subunit of the dolichol-phosphate mannose (DPM) synthase complex.
Protein Name Dolichol-Phosphate Mannosyltransferase Subunit 1
Dolichol-Phosphate Mannose Synthase Subunit 1
Dpm Synthase Subunit 1
Dolichyl-Phosphate Beta-D-Mannosyltransferase Subunit 1
Mannose-P-Dolichol Synthase Subunit 1
Mpd Synthase Subunit 1
Database Links Reactome: R-HSA-162699
Reactome: R-HSA-4717374
Reactome: R-HSA-4719360
Reactome: R-HSA-4719377
Cellular Localisation Endoplasmic Reticulum
Alternative Antibody Names Anti-Dolichol-Phosphate Mannosyltransferase Subunit 1 antibody
Anti-Dolichol-Phosphate Mannose Synthase Subunit 1 antibody
Anti-Dpm Synthase Subunit 1 antibody
Anti-Dolichyl-Phosphate Beta-D-Mannosyltransferase Subunit 1 antibody
Anti-Mannose-P-Dolichol Synthase Subunit 1 antibody
Anti-Mpd Synthase Subunit 1 antibody
Anti-DPM1 antibody

Information sourced from Uniprot.org

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