Anti-DPH2 antibody (120-330) (STJ119582)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DPH2 (120-330) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DPH2 |
| Gene ID | 1802 |
| Uniprot ID | DPH2_HUMAN |
| Immunogen Region | 120-330 |
| Immunogen Sequence | VAFVLRQRSVALELCVKAFE AQNPDPKAPVVLLSEPACAH ALEALATLLRPRYLDLLVSS PAFPQPVGSLSPEPMPLERF GRRFPLAPGRRLEEYGAFYV GGSKASPDPDLDPDLSRLLL GWAPGQPFSSCCPDTGKTQD EGARAGRLRARRRYLVERAR DARVVGLLAGTLGVAQHREA LAHLRNLTQAAGKRSYVLAL GRPTPAKLANF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 120-330 of human DPH2 (NP_001375.2). |
Additional Info
| Tissue Specificity | Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes. |
| Function | Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit. |
| Protein Name | 2-(3-Amino-3-Carboxypropylhistidine Synthase Subunit 2Diphthamide Biosynthesis Protein 2Diphtheria Toxin Resistance Protein 2S-Adenosyl-L-Methionine -L-Histidine 3-Amino-3-Carboxypropyltransferase 2 |
| Database Links | Reactome: R-HSA-5358493 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-2-(3-Amino-3-Carboxypropylhistidine Synthase Subunit 2 antibodyAnti-Diphthamide Biosynthesis Protein 2 antibodyAnti-Diphtheria Toxin Resistance Protein 2 antibodyAnti-S-Adenosyl-L-Methionine -L-Histidine 3-Amino-3-Carboxypropyltransferase 2 antibodyAnti-DPH2 antibodyAnti-DPH2L2 antibody |
Information sourced from Uniprot.org