Anti-DOT1L antibody (1-250) (STJ113755)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Dot1L/KMT4 (1-250) antibody for use in WB and ELISA in rat and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DOT1L |
| Gene ID | 84444 |
| Uniprot ID | DOT1L_HUMAN |
| Immunogen Region | 1-250 |
| Immunogen Sequence | MGEKLELRLKSPVGAEPAVY PWPLPVYDKHHDAAHEIIET IRWVCEEIPDLKLAMENYVL IDYDTKSFESMQRLCDKYNR AIDSIHQLWKGTTQPMKLNT RPSTGLLRHILQQVYNHSVT DPEKLNNYEPFSPEVYGETS FDLVAQMIDEIKMTDDDLFV DLGSGVGQVVLQVAAATNCK HHYGVEKADIPAKYAETMDR EFRKWMKWYGKKHAEYTLER GDFLSEEWRERIANTSVIF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Dot1L/KMT4 (NP_115871.1). |
Additional Info
| Function | Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. |
| Protein Name | Histone-Lysine N-Methyltransferase - H3 Lysine-79 SpecificDot1-Like ProteinHistone H3-K79 MethyltransferaseH3-K79-HmtaseLysine N-Methyltransferase 4 |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase - H3 Lysine-79 Specific antibodyAnti-Dot1-Like Protein antibodyAnti-Histone H3-K79 Methyltransferase antibodyAnti-H3-K79-Hmtase antibodyAnti-Lysine N-Methyltransferase 4 antibodyAnti-DOT1L antibodyAnti-KIAA1814 antibodyAnti-KMT4 antibody |
Information sourced from Uniprot.org