| Short Description : | Rabbit monoclonal DOK1 (382-481) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S2MR |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DOK1 |
| Gene ID: | 1796 |
| Uniprot ID: | DOK1_HUMAN |
| Immunogen Region: | 382-481 |
| Immunogen Sequence: | EPKDAWWCQARVKEEGYELP YNPATDDYAVPPPRSTKPLL APKPQGPAFPEPGTATGSGI KSHNSALYSQVQKSGASGSW DCGLSRVGTDKTGVKSEGST |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 382-481 of human DOK1 (Q99704). |
| Tissue Specificity | Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer. |
| Post Translational Modifications | Constitutively tyrosine-phosphorylated. Phosphorylated by TEC. Phosphorylated by LYN. Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS. |
| Function | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. |
| Protein Name | Docking Protein 1Downstream Of Tyrosine Kinase 1P62(DokPp62 |
| Database Links | Reactome: R-HSA-8849469Reactome: R-HSA-8853659 |
| Cellular Localisation | Isoform 1: CytoplasmNucleusIsoform 3: CytoplasmPerinuclear Region |
| Alternative Antibody Names | Anti-Docking Protein 1 antibodyAnti-Downstream Of Tyrosine Kinase 1 antibodyAnti-P62(Dok antibodyAnti-Pp62 antibodyAnti-DOK1 antibody |
Information sourced from Uniprot.org
