• Immunofluorescence analysis of HeLa cells using DOK1 antibody (STJ27654). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using DOK1 Antibody (STJ27654) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using DOK1 Antibody (STJ27654) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using DOK1 Antibody (STJ27654) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using DOK1 antibody (STJ27654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DOK1 antibody (1-280) (STJ27654)

SKU:
STJ27654

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DOK1 (1-280) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DOK1
Gene ID: 1796
Uniprot ID: DOK1_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DOK1 (NP_001372.1).
Immunogen Sequence: MDGAVMEGPLFLQSQRFGTK RWRKTWAVLYPASPHGVARL EFFDHKGSSSGGGRGSSRRL DCKVIRLAECVSVAPVTVET PPEPGATAFRLDTAQRSHLL AADAPSSAAWVQTLCRNAFP KGSWTLAPTDNPPKLSALEM LENSLYSPTWEGSQFWVTVQ RTEAAERCGLHGSYVLRVEA ERLTLLTVGAQSQILEPLLS WPYTLLRRYGRDKVMFSFEA GRRCPSGPGTFTFQTAQGN
Tissue Specificity Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
Post Translational Modifications Constitutively tyrosine-phosphorylated. Phosphorylated by TEC. Phosphorylated by LYN. Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.
Function DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Protein Name Docking Protein 1
Downstream Of Tyrosine Kinase 1
P62(Dok
Pp62
Database Links Reactome: R-HSA-8849469
Reactome: R-HSA-8853659
Cellular Localisation Isoform 1: Cytoplasm
Nucleus
Isoform 3: Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Docking Protein 1 antibody
Anti-Downstream Of Tyrosine Kinase 1 antibody
Anti-P62(Dok antibody
Anti-Pp62 antibody
Anti-DOK1 antibody

Information sourced from Uniprot.org

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