Anti-Dnmt3L antibody (375-421) {Biotin} (STJ506263)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationBiotin
IsotypeIgG
ImmunogenSynthetic peptide corresponding to amino acids within region 375-421on mouse DNA (cytosine-5)-methyltransferase 3-like protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-Dnmt3L (375-421) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IP/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.5-1.5 Mu g/Mu l |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:10, 000ICC 1:50-1:100IF 1:50-1:100IHC 1:50-1:100IP 1:200WB 1:500 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Dnmt3l |
| Gene ID | 54427 |
| Uniprot ID | DNM3L_MOUSE |
| Immunogen | Synthetic peptide corresponding to amino acids within region 375-421on mouse DNA (cytosine-5)-methyltransferase 3-like protein. |
| Immunogen Region | 375-421 |
Additional Info
| Function | Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression. |
| Protein Name | DnaCytosine-5-Methyltransferase 3-Like |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna antibodyAnti-Cytosine-5-Methyltransferase 3-Like antibodyAnti-Dnmt3l antibody |
Information sourced from Uniprot.org