• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g DNMT3A antibody (STJ11101751). Western blot was performed from the immunoprecipitate using DNMT3A antibody (STJ11101751) at a dilution of 1:1000.
  • Western blot analysis of lysates from wild type (WT) and DNMT3A knockout (KO) 293T cells, using [KO Validated] DNMT3A (STJ11101751) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of lysates from Mouse Thymus, using DNMT3A Rabbit monoclonal antibody (STJ11101751) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DNMT3A antibody (550-650) [S1MR] (STJ11101751)

SKU:
STJ11101751

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Dnmt3a (550-650) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNMT3A
Gene ID: 1788
Uniprot ID: DNM3A_HUMAN
Immunogen Region: 550-650
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human DNMT3A (Q9Y6K1).
Immunogen Sequence: GNNNCCRCFCVECVDLLVGP GAAQAAIKEDPWNCYMCGHK GTYGLLRRREDWPSRLQMFF ANNHDQEFDPPKVYPPVPAE KRKPIRVLSLFDGIATGLLV L
Tissue Specificity Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
Post Translational Modifications Sumoylated.sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. Auto-methylated at Cys-710: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell.
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on Cys-710 in absence of DNA.
Protein Name Dna
Cytosine-5-Methyltransferase 3a
Dnmt3a
Cysteine Methyltransferase Dnmt3a
Dna Methyltransferase Hsaiiia
Dna Mtase Hsaiiia
M.hsaiiia
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-3214858
Reactome: R-HSA-4655427
Reactome: R-HSA-5334118
Reactome: R-HSA-9710421
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Accumulates In The Major Satellite Repeats At Pericentric Heterochromatin
Alternative Antibody Names Anti-Dna antibody
Anti-Cytosine-5-Methyltransferase 3a antibody
Anti-Dnmt3a antibody
Anti-Cysteine Methyltransferase Dnmt3a antibody
Anti-Dna Methyltransferase Hsaiiia antibody
Anti-Dna Mtase Hsaiiia antibody
Anti-M.hsaiiia antibody
Anti-DNMT3A antibody

Information sourced from Uniprot.org

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