![Western blot analysis of lysates from wild type (WT) and DNMT3A knockout (KO) 293T cells, using [KO Validated] DNMT3A (STJ11101751) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90666/360663/STJ11101751_2__13130.1713125352.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNMT3A knockout (KO) 293T cells, using [KO Validated] DNMT3A (STJ11101751) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
Anti-DNMT3A antibody (550-650) [S1MR] (STJ11101751)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Dnmt3a (550-650) antibody for use in WB, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S1MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DNMT3A |
| Gene ID | 1788 |
| Uniprot ID | DNM3A_HUMAN |
| Immunogen Region | 550-650 |
| Immunogen Sequence | GNNNCCRCFCVECVDLLVGP GAAQAAIKEDPWNCYMCGHK GTYGLLRRREDWPSRLQMFF ANNHDQEFDPPKVYPPVPAE KRKPIRVLSLFDGIATGLLV L |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human DNMT3A (Q9Y6K1). |
Additional Info
| Tissue Specificity | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. |
| Post Translational Modifications | Sumoylated.sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. Auto-methylated at Cys-710: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell. |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on Cys-710 in absence of DNA. |
| Protein Name | DnaCytosine-5-Methyltransferase 3aDnmt3aCysteine Methyltransferase Dnmt3aDna Methyltransferase HsaiiiaDna Mtase HsaiiiaM.hsaiiia |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-3214858Reactome: R-HSA-4655427Reactome: R-HSA-5334118Reactome: R-HSA-9710421Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusChromosomeCytoplasmAccumulates In The Major Satellite Repeats At Pericentric Heterochromatin |
| Alternative Antibody Names | Anti-Dna antibodyAnti-Cytosine-5-Methyltransferase 3a antibodyAnti-Dnmt3a antibodyAnti-Cysteine Methyltransferase Dnmt3a antibodyAnti-Dna Methyltransferase Hsaiiia antibodyAnti-Dna Mtase Hsaiiia antibodyAnti-M.hsaiiia antibodyAnti-DNMT3A antibody |
Information sourced from Uniprot.org