Anti-Dnmt3a antibody [R03-8H4] (STJA0035185)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Dnmt3a
General Information
| Short Description | Rabbit monoclonal anti-Dnmt3a for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R03-8H4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DNMT3A |
| Gene ID | 1788 |
| Uniprot ID | DNM3A_HUMAN |
| Immunogen | A synthetic peptide of human Dnmt3a |
Additional Info
| Tissue Specificity | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. |
| Post Translational Modifications | Sumoylated.sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. Auto-methylated at Cys-710: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell. |
| Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on Cys-710 in absence of DNA. |
| Protein Name | DnaCytosine-5-Methyltransferase 3aDnmt3aCysteine Methyltransferase Dnmt3aDna Methyltransferase HsaiiiaDna Mtase HsaiiiaM.hsaiiia |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-3214858Reactome: R-HSA-4655427Reactome: R-HSA-5334118Reactome: R-HSA-9710421Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusChromosomeCytoplasmAccumulates In The Major Satellite Repeats At Pericentric Heterochromatin |
| Alternative Antibody Names | Anti-Dna antibodyAnti-Cytosine-5-Methyltransferase 3a antibodyAnti-Dnmt3a antibodyAnti-Cysteine Methyltransferase Dnmt3a antibodyAnti-Dna Methyltransferase Hsaiiia antibodyAnti-Dna Mtase Hsaiiia antibodyAnti-M.hsaiiia antibodyAnti-DNMT3A antibody |
Information sourced from Uniprot.org