Anti-Dnmt1 antibody [R08-4C7] (STJA0036274)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Dnmt1
General Information
| Short Description | Rabbit monoclonal anti-Dnmt1 for use in WB, IHC-P, ICC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-4C7 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DNMT1 |
| Gene ID | 1786 |
| Uniprot ID | DNMT1_HUMAN |
| Immunogen | A synthesized peptide derived from human Dnmt1 |
Additional Info
| Tissue Specificity | Ubiquitous.highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. |
| Post Translational Modifications | Sumoylated.sumoylation increases activity. Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity. Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 thereby increasing DNMT1 stability. Methylation at Lys-142 by SETD7 is necessary for the regulation of DNMT1 proteasomal degradation. Ubiquitinated by UHRF1.interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome. |
| Function | Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth. |
| Protein Name | DnaCytosine-5-Methyltransferase 1Dnmt1Cxxc-Type Zinc Finger Protein 9Dna Methyltransferase HsaiDna Mtase HsaiM.hsaiMcmt |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-427413Reactome: R-HSA-4655427Reactome: R-HSA-5334118Reactome: R-HSA-9701898Reactome: R-HSA-9710421Reactome: R-HSA-9725371 |
| Cellular Localisation | NucleusLocalized To The Perinucleolar Region |
| Alternative Antibody Names | Anti-Dna antibodyAnti-Cytosine-5-Methyltransferase 1 antibodyAnti-Dnmt1 antibodyAnti-Cxxc-Type Zinc Finger Protein 9 antibodyAnti-Dna Methyltransferase Hsai antibodyAnti-Dna Mtase Hsai antibodyAnti-M.hsai antibodyAnti-Mcmt antibodyAnti-DNMT1 antibodyAnti-AIM antibodyAnti-CXXC9 antibodyAnti-DNMT antibody |
Information sourced from Uniprot.org