• Western blot analysis of extracts of various cell lines, using DNMT1 antibody (STJ11102767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using DNMT1 antibody (STJ11102767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using DNMT1 antibody (STJ11102767) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using DNMT1 antibody (STJ11102767) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using DNMT1 antibody (STJ11102767) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g DNMT1 antibody (STJ11102767). Western blot was performed from the immunoprecipitate using DNMT1 antibody (STJ11102767) at a dilution of 1:1000.

Anti-DNMT1 antibody (1-124) (STJ11102767)

SKU:
STJ11102767

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNMT1 (1-124) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNMT1
Gene ID: 1786
Uniprot ID: DNMT1_HUMAN
Immunogen Region: 1-124
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of DNMT1 (NP_001370.1).
Immunogen Sequence: MPARTAPARVPTLAVPAISL PDDVRRRLKDLERDSLTEKE CVKEKLNLLHEFLQTEIKNQ LCDLETKLRKEELSEEGYLA KVKSLLNKDLSLENGAHAYN REVNGRLENGNQARSEARRV GMAD
Tissue Specificity Ubiquitous.highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Post Translational Modifications Sumoylated.sumoylation increases activity. Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G(2)/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity. Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability. Methylation at Lys-142 by SETD7 is necessary for the regulation of DNMT1 proteasomal degradation. Ubiquitinated by UHRF1.interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.
Function Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.
Protein Name Dna
Cytosine-5-Methyltransferase 1
Dnmt1
Cxxc-Type Zinc Finger Protein 9
Dna Methyltransferase Hsai
Dna Mtase Hsai
M.hsai
Mcmt
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-427413
Reactome: R-HSA-4655427
Reactome: R-HSA-5334118
Reactome: R-HSA-9710421
Cellular Localisation Nucleus
Localized To The Perinucleolar Region
Alternative Antibody Names Anti-Dna antibody
Anti-Cytosine-5-Methyltransferase 1 antibody
Anti-Dnmt1 antibody
Anti-Cxxc-Type Zinc Finger Protein 9 antibody
Anti-Dna Methyltransferase Hsai antibody
Anti-Dna Mtase Hsai antibody
Anti-M.hsai antibody
Anti-Mcmt antibody
Anti-DNMT1 antibody
Anti-AIM antibody
Anti-CXXC9 antibody
Anti-DNMT antibody

Information sourced from Uniprot.org

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