• Immunofluorescence analysis of L929 cells using Dynamin 3 Rabbit polyclonal antibody (STJ117342) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Dynamin 3 Rabbit polyclonal antibody (STJ117342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DNM3 antibody (410-555) (STJ117342)

SKU:
STJ117342

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNM3 (410-555) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNM3
Gene ID: 26052
Uniprot ID: DYN3_HUMAN
Immunogen Region: 410-555
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 410-555 of human Dynamin 3 (NP_001265181.1).
Immunogen Sequence: EAIVKKQIVKLKGPSLKSVD LVIQELINTVKKCTKKLANF PRLCEETERIVANHIREREG KTKDQVLLLIDIQVSYINTN HEDFIGFANAQQRSSQVHKK TTVGNQVIRKGWLTISNIGI MKGGSKGYWFVLTAESLSWY KDDENI
Function Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
Protein Name Dynamin-3
Dynamin - Testicular
T-Dynamin
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-177504
Reactome: R-HSA-2132295
Reactome: R-HSA-437239
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule-Associated
Alternative Antibody Names Anti-Dynamin-3 antibody
Anti-Dynamin - Testicular antibody
Anti-T-Dynamin antibody
Anti-DNM3 antibody
Anti-KIAA0820 antibody

Information sourced from Uniprot.org

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