• Western blot analysis of extracts of various cell lines, using DNM2 Antibody (STJ110200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunofluorescence analysis of NIH-3T3 cells using DNM2 Rabbit polyclonal antibody (STJ110200) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using DNM2 rabbit polyclonal antibody (STJ110200) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DNM2 Antibody (STJ110200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-DNM2 antibody (607-866) (STJ110200)

SKU:
STJ110200

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNM2 (607-866) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNM2
Gene ID: 1785
Uniprot ID: DYN2_HUMAN
Immunogen Region: 607-866
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 607-866 of human DNM2 (NP_004936.2).
Immunogen Sequence: EDVDSWKASFLRAGVYPEKD QAENEDGAQENTFSMDPQLE RQVETIRNLVDSYVAIINKS IRDLMPKTIMHLMINNTKAF IHHELLAYLYSSADQSSLME ESADQAQRRDDMLRMYHALK EALNIIGDISTSTVSTPVPP PVDDTWLQSASSHSPTPQRR PVSSIHPPGRPPAVRGPTPG PPLIPVPVGAAASFSAPPIP SRPGPQSVFANSDLFPAPPQ IPSRPVRIPPGIPPGVPSR
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by calcineurin/PP2. Phosphorylated on tyrosine residues after activation of SRC.
Function Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane.
Protein Name Dynamin-2
Database Links Reactome: R-HSA-166016
Reactome: R-HSA-177504
Reactome: R-HSA-190873
Reactome: R-HSA-196025
Reactome: R-HSA-203641
Reactome: R-HSA-2132295
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Reactome: R-HSA-437239
Reactome: R-HSA-8856828
Reactome: R-HSA-9031628
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Membrane
Clathrin-Coated Pit
Postsynaptic Density
Synapse
Midbody
Cell Projection
Phagocytic Cup
Cytoplasmic Vesicle
Phagosome Membrane
Peripheral Membrane Protein
Colocalizes With Cttn At The Basis Of Filopodia In Hippocampus Neuron Growth Zones
Microtubule-Associated
Also Found In The Postsynaptic Density Of Neuronal Cells
Co-Localizes With Pik3c3 And Rab5a To The Nascent Phagosome
Alternative Antibody Names Anti-Dynamin-2 antibody
Anti-DNM2 antibody
Anti-DYN2 antibody

Information sourced from Uniprot.org

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