![Western blot analysis of lysates from wild type (WT) and DNAJC9 knockout (KO) 293T cells, using [KO Validated] DNAJC9 Rabbit polyclonal antibody (STJ11100726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89664/358161/STJ11100726_1__26294.1713122463.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNAJC9 knockout (KO) 293T cells, using [KO Validated] DNAJC9 Rabbit polyclonal antibody (STJ11100726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and DNAJC9 knockout (KO) 293T cells, using [KO Validated] DNAJC9 Rabbit polyclonal antibody (STJ11100726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89664/358161/STJ11100726_1__26294.1713122463.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNAJC9 knockout (KO) 293T cells, using [KO Validated] DNAJC9 Rabbit polyclonal antibody (STJ11100726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Short Description : | Rabbit polyclonal DNAJC9 (1-260) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DNAJC9 |
| Gene ID: | 23234 |
| Uniprot ID: | DNJC9_HUMAN |
| Immunogen Region: | 1-260 |
| Immunogen Sequence: | MGLLDLCEEVFGTADLYRVL GVRREASDGEVRRGYHKVSL QVHPDRVGEGDKEDATRRFQ ILGKVYSVLSDREQRAVYDE QGTVDEDSPVLTQDRDWEAY WRLLFKKISLEDIQAFEKTY KGSEEELADIKQAYLDFKGD MDQIMESVLCVQYTEEPRIR NIIQQAIDAGEVPSYNAFVK ESKQKMNARKRRAQEEAKEA EMSRKELGLDEGVDSLKAAI QSRQKDRQKEMDNFLAQME |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DNAJC9 (NP_056005.1). |
| Tissue Specificity | Expressed in heart, placenta, liver, skeletal muscle, kidney, pancreas, thymus, ovary, colon and peripheral blood. |
| Function | Acts as a dual histone chaperone and heat shock co-chaperone. As a histone chaperone, forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers.and may thereby assist MCM2 in histone H3-H4 heterodimer recognition and facilitate the assembly of histones into nucleosomes. May also act as a histone co-chaperone together with TONSL. May recruit histone chaperones ASF1A, NASP and SPT2 to histone H3-H4 heterodimers. Also plays a role as co-chaperone of the HSP70 family of molecular chaperone proteins, such as HSPA1A, HSPA1B and HSPA8. As a co-chaperone, may play a role in the recruitment of HSP70-type molecular chaperone machinery to histone H3-H4 substrates, thereby maintaining the histone structural integrity. Exhibits activity to assemble histones onto DNA in vitro. |
| Protein Name | Dnaj Homolog Subfamily C Member 9Hdjc9Dnaj Protein Sb73 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCell MembranePredominantly NuclearTranslocates To The Cytoplasm And Membrane After Heat Shock |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily C Member 9 antibodyAnti-Hdjc9 antibodyAnti-Dnaj Protein Sb73 antibodyAnti-DNAJC9 antibody |
Information sourced from Uniprot.org
