• Western blot analysis of extracts of various cell lines, using DNAJC2 antibody (STJ23400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DNAJC2 antibody (1-140) (STJ23400)

SKU:
STJ23400

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNAJC2 (1-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNAJC2
Gene ID: 27000
Uniprot ID: DNJC2_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human DNAJC2 (NP_055192.1).
Immunogen Sequence: MLLLPSAADGRGTAITHALT SASTLCQVEPVGRWFEAFVK RRNRNASASFQELEDKKELS EESEDEELQLEEFPMLKTLD PKDWKNQDHYAVLGLGHVRY KATQRQIKAAHKAMVLKHHP DKRKAAGEPIKEGDNDYFTC
Tissue Specificity Widely expressed.
Post Translational Modifications Phosphorylated in M (mitotic) phase.
Function Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation.
Protein Name Dnaj Homolog Subfamily C Member 2
M-Phase Phosphoprotein 11
Zuotin-Related Factor 1 Cleaved Into - Dnaj Homolog Subfamily C Member 2 - N-Terminally Processed
Database Links Reactome: R-HSA-3371453
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Alternative Antibody Names Anti-Dnaj Homolog Subfamily C Member 2 antibody
Anti-M-Phase Phosphoprotein 11 antibody
Anti-Zuotin-Related Factor 1 Cleaved Into - Dnaj Homolog Subfamily C Member 2 - N-Terminally Processed antibody
Anti-DNAJC2 antibody
Anti-MPHOSPH11 antibody
Anti-MPP11 antibody
Anti-ZRF1 antibody

Information sourced from Uniprot.org

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