• STJ72616 (1µg/ml) staining of Human Liver (A) , Kidney (B) and-Mouse Liver (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72616 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72616 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72616 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-DNAJB9 antibody (61-75) (STJ72616)

SKU:
STJ72616-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DNAJB9 (61-75) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DNAJB9
Gene ID: 4189
Uniprot ID: DNJB9_HUMAN
Immunogen Region: 61-75
Accession Number: NP_036460.1
Immunogen Sequence: PDAEAKFREIAEAYE
Function Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins. In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1. Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production.
Protein Name Dnaj Homolog Subfamily B Member 9
Endoplasmic Reticulum Dna J Domain-Containing Protein 4
Er-Resident Protein Erdj4
Erdj4
Microvascular Endothelial Differentiation Gene 1 Protein
Mdg-1
Database Links Reactome: R-HSA-381038
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Dnaj Homolog Subfamily B Member 9 antibody
Anti-Endoplasmic Reticulum Dna J Domain-Containing Protein 4 antibody
Anti-Er-Resident Protein Erdj4 antibody
Anti-Erdj4 antibody
Anti-Microvascular Endothelial Differentiation Gene 1 Protein antibody
Anti-Mdg-1 antibody
Anti-DNAJB9 antibody
Anti-MDG1 antibody
Anti-UNQ743 antibody
Anti-PRO1471 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance