| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-DNA Polymerase beta (236-335) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S1MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLB |
| Gene ID: |
5423 |
| Uniprot ID: |
DPOLB_HUMAN |
| Immunogen Region: |
236-335 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 236-335 of human DNA Polymerase beta (P06746). |
| Immunogen Sequence: |
MGVCQLPSKNDEKEYPHRRI DIRLIPKDQYYCGVLYFTGS DIFNKNMRAHALEKGFTINE YTIRPLGVTGVAGEPLPVDS EKDIFDYIQWKYREPKDRSE |
| Post Translational Modifications | Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation. |
| Function | Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase).through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase. |
| Protein Name | Dna Polymerase Beta5'-Deoxyribose-Phosphate Lyase5'-Drp LyaseAp Lyase |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-110373Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5651801Reactome: R-HSA-5689880Reactome: R-HSA-73930 |
| Cellular Localisation | NucleusCytoplasmCytoplasmic In Normal ConditionsTranslocates To The Nucleus Following Dna Damage |
| Alternative Antibody Names | Anti-Dna Polymerase Beta antibodyAnti-5'-Deoxyribose-Phosphate Lyase antibodyAnti-5'-Drp Lyase antibodyAnti-Ap Lyase antibodyAnti-POLB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
