Anti-DNA Ligase IV antibody [R08-3B4] (STJA0034252)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human DNA Ligase IV
General Information
| Short Description | Rabbit monoclonal anti-DNA Ligase IV for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-3B4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | LIG4 |
| Gene ID | 3981 |
| Uniprot ID | DNLI4_HUMAN |
| Immunogen | Recombinant protein of human DNA Ligase IV |
Additional Info
| Tissue Specificity | Testis, thymus, prostate and heart. |
| Function | DNA ligase involved in DNA non-homologous end joining (NHEJ).required for double-strand break (DSB) repair and V(D)J recombination. Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed. Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps. Forms a subcomplex with XRCC4.the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. LIG4 regulates nuclear localization of XRCC4. |
| Protein Name | Dna Ligase 4Dna Ligase IvPolydeoxyribonucleotide Synthase Atp 4 |
| Database Links | Reactome: R-HSA-164843Reactome: R-HSA-5693571 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Ligase 4 antibodyAnti-Dna Ligase Iv antibodyAnti-Polydeoxyribonucleotide Synthase Atp 4 antibodyAnti-LIG4 antibody |
Information sourced from Uniprot.org