Anti-DMP1 antibody (Internal) (STJ71708)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-DMP1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 55kDa band observed in Human Ileum and Kidney lysates (calculated MW of 55.8kDa according to NP_004398.1 and of 54.1 according to NP_001073380.1). Recommended concentration: 1-3 |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | DMP1 |
| Gene ID | 1758 |
| Uniprot ID | DMP1_HUMAN |
| Accession Number | NP_004398.1; NP_001073380.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | ENSEQANTQDSG |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_004398.1; NP_001073380.1). |
Additional Info
| Post Translational Modifications | Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. |
| Function | May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. |
| Protein Name | Dentin Matrix Acidic Phosphoprotein 1Dmp-1Dentin Matrix Protein 1 |
| Database Links | Reactome: R-HSA-3000178Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | NucleusCytoplasmSecretedExtracellular SpaceExtracellular MatrixIn Proliferating Preosteoblasts It Is NuclearDuring Early Maturation Stage Is Cytoplasmic And In Mature Osteoblast Localizes In The Mineralized MatrixExport From The Nucleus Of Differentiating Osteoblast Is Triggered By The Release Of Calcium From Intracellular Stores Followed By A Massive Influx Of This Pool Of Calcium Into The Nucleus |
| Alternative Antibody Names | Anti-Dentin Matrix Acidic Phosphoprotein 1 antibodyAnti-Dmp-1 antibodyAnti-Dentin Matrix Protein 1 antibodyAnti-DMP1 antibody |
Information sourced from Uniprot.org