• Western blot analysis of extracts of Rat testis, using DMC1 antibody (STJ23393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using DMC1 antibody (STJ23393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DMC1 antibody (1-260) (STJ23393)

SKU:
STJ23393

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DMC1 (1-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DMC1
Gene ID: 11144
Uniprot ID: DMC1_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DMC1 (NP_008999.2).
Immunogen Sequence: MKEDQVVAEEPGFQDEEESL FQDIDLLQKHGINVADIKKL KSVGICTIKGIQMTTRRALC NVKGLSEAKVDKIKEAANKL IEPGFLTAFEYSEKRKMVFH ITTGSQEFDKLLGGGIESMA ITEAFGEFRTGKTQLSHTLC VTAQLPGAGGYPGGKIIFID TENTFRPDRLRDIADRFNVD HDAVLDNVLYARAYTSEHQM ELLDYVAAKFHEEAGIFKLL IIDSIMALFRVDFSGRGEL
Function Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Protein Name Meiotic Recombination Protein Dmc1/Lim15 Homolog
Database Links Reactome: R-HSA-912446
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Meiotic Recombination Protein Dmc1/Lim15 Homolog antibody
Anti-DMC1 antibody
Anti-DMC1H antibody
Anti-LIM15 antibody

Information sourced from Uniprot.org

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