• Immunofluorescence analysis of HeLa cells using DLGAP5 antibody (STJ115536). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DLGAP5 antibody (STJ115536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DLGAP5 antibody (547-846) (STJ115536)

SKU:
STJ115536

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DLGAP5 (547-846) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLGAP5
Gene ID: 9787
Uniprot ID: DLGP5_HUMAN
Immunogen Region: 547-846
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 547-846 of human DLGAP5 (NP_055565.3).
Immunogen Sequence: KNVFRKKVVSGIASKPKQDD AGRIAARNRLAAIKNAMRER IRQEECAETAVSVIPKEVDK IVFDAGFFRVESPVKLFSGL SVSSEGPSQRLGTPKSVNKA VSQSRNEMGIPQQTTSPENA GPQNTKSEHVKKTLFLSIPE SRSSIEDAQCPGLPDLIEEN HVVNKTDLKVDCLSSERMSL PLLAGGVADDINTNKKEGIS DVVEGMELNSSITSQDVLMS SPEKNTASQNSILEEGETK
Tissue Specificity Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
Post Translational Modifications Ubiquitinated, leading to its degradation. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
Function Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
Protein Name Disks Large-Associated Protein 5
Dap-5
Discs Large Homolog 7
Disks Large-Associated Protein Dlg7
Hepatoma Up-Regulated Protein
Hurp
Database Links Reactome: R-HSA-9013508
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Spindle
Localizes To The Spindle In Mitotic Cells
Colocalizes With Cdh1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells
Alternative Antibody Names Anti-Disks Large-Associated Protein 5 antibody
Anti-Dap-5 antibody
Anti-Discs Large Homolog 7 antibody
Anti-Disks Large-Associated Protein Dlg7 antibody
Anti-Hepatoma Up-Regulated Protein antibody
Anti-Hurp antibody
Anti-DLGAP5 antibody
Anti-DLG7 antibody
Anti-KIAA0008 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance