Anti-DLG1 antibody (Internal) (STJ71290)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-DLG1 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/IF/FC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary testing showed a band at approx 150kDa in lysate of cell line Kelly after 0.3µg/ml antibody staining (calculated MW of 103kDa according to NP_004078.2). This molecular weig |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | DLG1 |
| Gene ID | 1739 |
| Uniprot ID | DLG1_HUMAN |
| Accession Number | NP_001091894.1; NP_004078.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | NSKTRDKGEIPDD |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001091894.1and NP_004078.2). |
Additional Info
| Post Translational Modifications | Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A. Ubiquitinated.by MARCHF2 which results in its degradation. |
| Function | Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion. May also play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane. |
| Protein Name | Disks Large Homolog 1Synapse-Associated Protein 97Sap-97Sap97Hdlg |
| Database Links | Reactome: R-HSA-399719Reactome: R-HSA-438066Reactome: R-HSA-442982Reactome: R-HSA-447038Reactome: R-HSA-451308Reactome: R-HSA-5673001Reactome: R-HSA-8849932Reactome: R-HSA-9609736Reactome: R-HSA-9617324Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinBasolateral Cell MembraneEndoplasmic Reticulum MembranePostsynaptic DensitySynapseSarcolemmaApical Cell MembraneCell JunctionCytoplasmColocalizes With Epb41 At Regions Of Intercellular ContactsBasolateral In Epithelial CellsMay Also Associate With Endoplasmic Reticulum MembranesMainly Found In Neurons SomaModerately Found At Postsynaptic Densities |
| Alternative Antibody Names | Anti-Disks Large Homolog 1 antibodyAnti-Synapse-Associated Protein 97 antibodyAnti-Sap-97 antibodyAnti-Sap97 antibodyAnti-Hdlg antibodyAnti-DLG1 antibody |
Information sourced from Uniprot.org