Anti-DLD antibody (401-500) [S0MR] (STJ11102300)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal DLDH (401-500) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S0MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DLD |
| Gene ID | 1738 |
| Uniprot ID | DLDH_HUMAN |
| Immunogen Region | 401-500 |
| Immunogen Sequence | WVGKSEEQLKEEGIEYKVGK FPFAANSRAKTNADTDGMVK ILGQKSTDRVLGAHILGPGA GEMVNEAALALEYGASCEDI ARVCHAHPTLSEAFREANLA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 401-500 of human DLDH/DLD (P09622). |
Additional Info
| Post Translational Modifications | Tyrosine phosphorylated. |
| Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. |
| Protein Name | Dihydrolipoyl Dehydrogenase - MitochondrialDihydrolipoamide DehydrogenaseGlycine Cleavage System L Protein |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-6783984Reactome: R-HSA-70895Reactome: R-HSA-9837999Reactome: R-HSA-9853506Reactome: R-HSA-9858328Reactome: R-HSA-9859138Reactome: R-HSA-9861559Reactome: R-HSA-9865113Reactome: R-HSA-9907570Reactome: R-HSA-9912481Reactome: R-HSA-9912529 |
| Cellular Localisation | Mitochondrion MatrixNucleusCell ProjectionCiliumFlagellumCytoplasmic VesicleSecretory VesicleAcrosomeMainly Localizes In The MitochondrionA Small Fraction Localizes To The NucleusWhere The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative Antibody Names | Anti-Dihydrolipoyl Dehydrogenase - Mitochondrial antibodyAnti-Dihydrolipoamide Dehydrogenase antibodyAnti-Glycine Cleavage System L Protein antibodyAnti-DLD antibodyAnti-GCSL antibodyAnti-LAD antibodyAnti-PHE3 antibody |
Information sourced from Uniprot.org