• Western blot analysis of extracts of various cell lines, using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of DLDH/DLD in paraffin-embedded human colon carcinoma tissue using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DLDH/DLD in paraffin-embedded human esophagus tissue using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DLDH/DLD in paraffin-embedded human kidney tissue using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DLDH/DLD in paraffin-embedded human liver tissue using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DLDH/DLD in paraffin-embedded human tonsil tissue using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DLDH/DLD in paraffin-embedded mouse kidney tissue using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of DLDH/DLD in paraffin-embedded rat spleen tissue using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH-3T3 cells using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using DLDH/DLD rabbit monoclonal antibody (STJ11102300) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DLD antibody (401-500) [S0MR] (STJ11102300)

SKU:
STJ11102300

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DLDH (401-500) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLD
Gene ID: 1738
Uniprot ID: DLDH_HUMAN
Immunogen Region: 401-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 401-500 of human DLDH/DLD (P09622).
Immunogen Sequence: WVGKSEEQLKEEGIEYKVGK FPFAANSRAKTNADTDGMVK ILGQKSTDRVLGAHILGPGA GEMVNEAALALEYGASCEDI ARVCHAHPTLSEAFREANLA
Post Translational Modifications Tyrosine phosphorylated.
Function Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Protein Name Dihydrolipoyl Dehydrogenase - Mitochondrial
Dihydrolipoamide Dehydrogenase
Glycine Cleavage System L Protein
Database Links Reactome: R-HSA-204174
Reactome: R-HSA-389661
Reactome: R-HSA-5362517
Reactome: R-HSA-6783984
Reactome: R-HSA-70268
Reactome: R-HSA-70895
Reactome: R-HSA-71064
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Matrix
Nucleus
Cell Projection
Cilium
Flagellum
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Mainly Localizes In The Mitochondrion
A Small Fraction Localizes To The Nucleus
Where The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation
Alternative Antibody Names Anti-Dihydrolipoyl Dehydrogenase - Mitochondrial antibody
Anti-Dihydrolipoamide Dehydrogenase antibody
Anti-Glycine Cleavage System L Protein antibody
Anti-DLD antibody
Anti-GCSL antibody
Anti-LAD antibody
Anti-PHE3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance