| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-DIXDC1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 60kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 53.9kDa according to NP_219493.1). Recommended concentration: 0.5-2µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DIXDC1 |
| Gene ID: | 85458 |
| Uniprot ID: | DIXC1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_001033043.1; NP_219493.1 |
| Specificity: | This antibody is expected to recognize both isoforms (NP_001033043.1; NP_219493.1). |
| Immunogen Sequence: | DVSYHQVDLERE |
| Post Translational Modifications | Phosphorylated on tyrosine and serine residues. Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation. |
| Function | Positive effector of the Wnt signaling pathway.activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2. |
| Protein Name | DixinCoiled-Coil Protein Dix1Coiled-Coil-Dix1Dix Domain-Containing Protein 1 |
| Cellular Localisation | Cell JunctionFocal AdhesionCytoplasmCytoskeletonStress FiberColocalizes With Gamma-Tubulin At The CentrosomeBoth During Interphase And MitosisAssociated With Actin Stress Fiber At The Filament EndsIsoform 2: CytoplasmNot Associated With Stress Fibers |
| Alternative Antibody Names | Anti-Dixin antibodyAnti-Coiled-Coil Protein Dix1 antibodyAnti-Coiled-Coil-Dix1 antibodyAnti-Dix Domain-Containing Protein 1 antibodyAnti-DIXDC1 antibodyAnti-CCD1 antibodyAnti-KIAA1735 antibody |
Information sourced from Uniprot.org
