Western blot analysis of extracts of various cell lines, using DIO3 antibody (STJ28980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DIO3 antibody (70-170) (STJ28980)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ28980
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General Information

Short DescriptionRabbit polyclonal DIO3 (70-170) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolDIO3
Gene ID1735
Uniprot IDIOD3_HUMAN
Immunogen Region70-170
Immunogen SequenceHFLGRRRRGQPEPEVELNSE GEEVPPDDPPICVSDDNRLC TLASLKAVWHGQKLDFFKQA HEGGPAPNSEVVLPDGFQSQ HILDYAQGNRPLVLNFGSCT U
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 70-170 of human DIO3 (NP_001353.4).

Additional Info

Tissue Specificity Expressed in placenta and several fetal tissues.
Function Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,3',5'-triiodothyronine (rT3), 3,5,3'-triiodothyronine (T3) to 3,3'-diiodothyronine (3,3'-T2), 3,5-diiodothyronine (3,5-T2) to 3-monoiodothyronine (3-T1), rT3 to 3',5'-diiodothyronine (3',5'-T2) and 3,3'-T2 to 3'-monoiodothyronine (3'-T1) via inner-ring deiodination (IRD). Catalyzes the deiodination of 3-T1 to L-thyronine (T0) via outer-ring deiodination (ORD). Catalyzes the tyrosyl ring deiodinations of 3,3',5,5'-tetraiodothyronamine, 3,3',5'-triiodothyronamine, 3,5,3'-triiodothyronamine, 3,5-diiodothyronamine, 3,3'-diiodothyronamine and 3-iodothyronamine.
Protein Name Thyroxine 5-Deiodinase
5diii
Dioiii
Type 3 Di
Type Iii Iodothyronine Deiodinase
Database Links Reactome: R-HSA-350864
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Endosome Membrane
Alternative Antibody Names Anti-Thyroxine 5-Deiodinase antibody
Anti-5diii antibody
Anti-Dioiii antibody
Anti-Type 3 Di antibody
Anti-Type Iii Iodothyronine Deiodinase antibody
Anti-DIO3 antibody
Anti-ITDI3 antibody
Anti-TXDI3 antibody

Information sourced from Uniprot.org

Citations

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