Anti-DIAPH3 antibody (630-849) (STJ112389)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DIAPH3 (630-849) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DIAPH3 |
| Gene ID | 81624 |
| Uniprot ID | DIAP3_HUMAN |
| Immunogen Region | 630-849 |
| Immunogen Sequence | KYPDILNFVDDLEPLDKASK VSVETLEKNLRQMGRQLQQL EKELETFPPPEDLHDKFVTK MSRFVISAKEQYETLSKLHE NMEKLYQSIIGYYAIDVKKV SVEDFLTDLNNFRTTFMQAI KENIKKREAEEKEKRVRIAK ELAERERLERQQKKKRLLEM KTEGDETGVMDNLLEALQSG AAFRDRRKRTPMPKDVRQSL SPMSQRPVLKVCNHGNKPYL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 630-849 of human DIAPH3 (NP_112194.2). |
Additional Info
| Post Translational Modifications | Ubiquitinated. |
| Function | Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity. |
| Protein Name | Protein Diaphanous Homolog 3Diaphanous-Related Formin-3Drf3Mdia2 |
| Database Links | Reactome: R-HSA-5663220Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013406Reactome: R-HSA-9013408Reactome: R-HSA-9013409Reactome: R-HSA-9013423Reactome: R-HSA-9035034 |
| Cellular Localisation | CytoplasmNucleusDuring MitosisCo-Localizes With The Actin-Rich Cleavage Furrow And With The Microtubule-Rich Central Spindle During CytokinesisShuttles Between The Cytoplasm And The Nucleus |
| Alternative Antibody Names | Anti-Protein Diaphanous Homolog 3 antibodyAnti-Diaphanous-Related Formin-3 antibodyAnti-Drf3 antibodyAnti-Mdia2 antibodyAnti-DIAPH3 antibodyAnti-DIAP3 antibody |
Information sourced from Uniprot.org