Anti-DHH antibody (STJA0009323)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human DHH
General Information
| Short Description | Rabbit polyclonal anti-Desert hedgehog protein for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DHH |
| Gene ID | 50846 |
| Uniprot ID | DHH_HUMAN |
| Immunogen | Synthesized peptide derived from human DHH |
| Specificity | This antibody detects endogenous levels of DHH at Human, Mouse |
Additional Info
| Function | Desert hedgehog protein: The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (N-product and C-product) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product. Both activities occur in the endoplasmic reticulum. Functions in cell-cell mediated juxtacrine signaling. Promotes endothelium integrity. Binds to PTCH1 receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes in endothelial cells. In Schwann cells, controls the development of the peripheral nerve sheath and the transition of mesenchymal cells to form the epithelium-like structure of the perineurial tube. Desert hedgehog protein N-product: The dually lipidated desert hedgehog protein N-product is essential for a variety of patterning events during development. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Required for normal testis development and spermatogenesis, namely for the formation of adult-type Leydig cells and normal development of peritubular cells and seminiferous tubules. Activates primary cilia signaling on neighboring valve interstitial cells through a paracrine mechanism. May induce motor neurons in lateral neural tube and may have a polarizing activity. Prevents the desert hedgehog protein precursor binding to PTCH1. |
| Protein Name | Desert Hedgehog ProteinDhhHhg-3 Cleaved Into - Desert Hedgehog Protein N-ProductDhh-N |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-5358346Reactome: R-HSA-5362798Reactome: R-HSA-5632681Reactome: R-HSA-5632684Reactome: R-HSA-5635838Reactome: R-HSA-5658034Reactome: R-HSA-9690406 |
| Cellular Localisation | Desert Hedgehog Protein N-Product: Cell MembraneLipid-AnchorDesert Hedgehog Protein: Endoplasmic Reticulum MembraneGolgi Apparatus MembraneSecretedCell MembraneCo-Localizes With Hhat In The Er And Golgi Membrane |
| Alternative Antibody Names | Anti-Desert Hedgehog Protein antibodyAnti-Dhh antibodyAnti-Hhg-3 Cleaved Into - Desert Hedgehog Protein N-Product antibodyAnti-Dhh-N antibodyAnti-DHH antibody |
Information sourced from Uniprot.org