Anti-DHCR24 antibody (227-516) (STJ27355)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DHCR24 (227-516) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DHCR24 |
| Gene ID | 1718 |
| Uniprot ID | DHC24_HUMAN |
| Immunogen Region | 227-516 |
| Immunogen Sequence | AEIRIIPAKKYVKLRFEPVR GLEAICAKFTHESQRQENHF VEGLLYSLDEAVIMTGVMTD EAEPSKLNSIGNYYKPWFFK HVENYLKTNREGLEYIPLRH YYHRHTRSIFWELQDIIPFG NNPIFRYLFGWMVPPKISLL KLTQGETLRKLYEQHHVVQD MLVPMKCLQQALHTFQNDIH VYPIWLCPFILPSQPGLVHP KGNEAELYIDIGAYGEPRVK HFEARSCMRQLEKFVRSVH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 227-516 of human DHCR24 (NP_055577.1). |
Additional Info
| Tissue Specificity | Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex. |
| Function | Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. In addition to its cholesterol-synthesizing activity, can protect cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis. |
| Protein Name | Delta(24-Sterol Reductase24-Dehydrocholesterol Reductase3-Beta-Hydroxysterol Delta-24-ReductaseDiminuto/Dwarf1 HomologSeladin-1 |
| Database Links | Reactome: R-HSA-191273Reactome: R-HSA-6807047Reactome: R-HSA-6807062 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinGolgi Apparatus Membrane |
| Alternative Antibody Names | Anti-Delta(24-Sterol Reductase antibodyAnti-24-Dehydrocholesterol Reductase antibodyAnti-3-Beta-Hydroxysterol Delta-24-Reductase antibodyAnti-Diminuto/Dwarf1 Homolog antibodyAnti-Seladin-1 antibodyAnti-DHCR24 antibodyAnti-KIAA0018 antibody |
Information sourced from Uniprot.org