| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DGUOK |
| Gene ID: | 1716 |
| Uniprot ID: | DGUOK_HUMAN |
| Immunogen Region: | 40-277 |
| Immunogen Sequence: | RLSIEGNIAVGKSTFVKLLT KTYPEWHVATEPVATWQNIQ AAGTQKACTAQSLGNLLDMM YREPARWSYTFQTFSFLSRL KVQLEPFPEKLLQARKPVQI FERSVYSDRYIFAKNLFENG SLSDIEWHIYQDWHSFLLWE FASRITLHGFIYLQASPQVC LKRLYQRAREEEKGIELAYL EQLHGQHEAWLIHKTTKLHF EALMNIPVLVLDVNDDFSEE VTKQEDLMREVNTFVKNL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-277 of human DGUOK (NP_550438.1). |
| Tissue Specificity | Ubiquitous. Highest expression in muscle, brain, liver and lymphoid tissues. |
| Function | Phosphorylates deoxyguanosine and deoxyadenosine in the mitochondrial matrix, with the highest efficiency for deoxyguanosine. In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on DGUOK and TK2. Phosphorylates certain nucleoside analogs. Widely used as target of antiviral and chemotherapeutic agents. |
| Protein Name | Deoxyguanosine Kinase - MitochondrialDeoxyadenosine Kinase - Mitochondrial |
| Database Links | Reactome: R-HSA-74217 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Deoxyguanosine Kinase - Mitochondrial antibodyAnti-Deoxyadenosine Kinase - Mitochondrial antibodyAnti-DGUOK antibodyAnti-DGK antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CBR1 knockout (KO) A-549 cells, using [KO Validated] CBR1 Rabbit polyclonal antibody (STJ11100047) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89119/357571/STJ11100047_1__35791.1713121699.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CBR1 knockout (KO) A-549 cells, using [KO Validated] CBR1 Rabbit polyclonal antibody (STJ11100047) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
