| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DGUOK (40-277) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DGUOK |
| Gene ID: |
1716 |
| Uniprot ID: |
DGUOK_HUMAN |
| Immunogen Region: |
40-277 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 40-277 of human DGUOK (NP_550438.1). |
| Immunogen Sequence: |
RLSIEGNIAVGKSTFVKLLT KTYPEWHVATEPVATWQNIQ AAGTQKACTAQSLGNLLDMM YREPARWSYTFQTFSFLSRL KVQLEPFPEKLLQARKPVQI FERSVYSDRYIFAKNLFENG SLSDIEWHIYQDWHSFLLWE FASRITLHGFIYLQASPQVC LKRLYQRAREEEKGIELAYL EQLHGQHEAWLIHKTTKLHF EALMNIPVLVLDVNDDFSEE VTKQEDLMREVNTFVKNL |
| Tissue Specificity | Ubiquitous. Highest expression in muscle, brain, liver and lymphoid tissues. |
| Function | Phosphorylates deoxyguanosine and deoxyadenosine in the mitochondrial matrix, with the highest efficiency for deoxyguanosine. In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on DGUOK and TK2. Phosphorylates certain nucleoside analogs. Widely used as target of antiviral and chemotherapeutic agents. |
| Protein Name | Deoxyguanosine Kinase - MitochondrialDeoxyadenosine Kinase - Mitochondrial |
| Database Links | Reactome: R-HSA-74217 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Deoxyguanosine Kinase - Mitochondrial antibodyAnti-Deoxyadenosine Kinase - Mitochondrial antibodyAnti-DGUOK antibodyAnti-DGK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and DGUOK knockout (KO) 293T cells, using [KO Validated] DGUOK Rabbit polyclonal antibody (STJ11100802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89738/358215/STJ11100802_1__20201.1713122540.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DGUOK knockout (KO) 293T cells, using [KO Validated] DGUOK Rabbit polyclonal antibody (STJ11100802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunofluorescence analysis of RAW264. 7 cells using [KO Validated] Caspase-3 Rabbit polyclonal antibody (STJ11101178) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/90100/358863/STJ11101178_1__46120.1713123228.jpg?c=1 "Immunofluorescence analysis of RAW264. 7 cells using [KO Validated] Caspase-3 Rabbit polyclonal antibody (STJ11101178) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
