Anti-DGKZ antibody (STJA0037901)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human DGKZ
STJA0037901
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General Information

Short DescriptionRabbit monoclonal anti-DGKZ for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC-P
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Purified
Dilution RangeIHC 1:100-1:200
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolDGKZ
Gene ID8525
Uniprot IDDGKZ_HUMAN
ImmunogenRecombinant protein of human DGKZ

Additional Info

Tissue Specificity Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 2: Predominantly expressed in muscle.
Post Translational Modifications Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.
Function Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group. The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol. By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization. Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane. Isoform 1: Regulates RASGRP1 activity. Isoform 2: Does not regulate RASGRP1 activity.
Protein Name Diacylglycerol Kinase Zeta
Dag Kinase Zeta
Diglyceride Kinase Zeta
Dgk-Zeta
Database Links Reactome: R-HSA-114508
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Cell Membrane
Cell Projection
Lamellipodium
Alternative Antibody Names Anti-Diacylglycerol Kinase Zeta antibody
Anti-Dag Kinase Zeta antibody
Anti-Diglyceride Kinase Zeta antibody
Anti-Dgk-Zeta antibody
Anti-DGKZ antibody
Anti-DAGK6 antibody

Information sourced from Uniprot.org

Citations

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