Anti-DGKZ antibody (601-650 aa) (STJ92700)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human DGKZ at the amino acid range 601-650
General Information
| Short Description | Rabbit polyclonal anti-Diacylglycerol kinase zeta (601-650 aa) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DGKZ |
| Gene ID | 8525 |
| Uniprot ID | DGKZ_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human DGKZ at the amino acid range 601-650 |
| Immunogen Region | 601-650 aa |
| Specificity | DGK-Zeta Polyclonal Antibody detects endogenous levels of DGK-Zeta protein. |
Additional Info
| Post Translational Modifications | Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta. |
| Function | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group. The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol. By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization. Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane. Isoform 1: Regulates RASGRP1 activity. Isoform 2: Does not regulate RASGRP1 activity. |
| Protein Name | Diacylglycerol Kinase ZetaDag Kinase ZetaDiglyceride Kinase ZetaDgk-Zeta |
| Database Links | Reactome: R-HSA-114508 |
| Cellular Localisation | NucleusCytoplasmCytosolCell MembraneCell ProjectionLamellipodium |
| Alternative Antibody Names | Anti-Diacylglycerol Kinase Zeta antibodyAnti-Dag Kinase Zeta antibodyAnti-Diglyceride Kinase Zeta antibodyAnti-Dgk-Zeta antibodyAnti-DGKZ antibodyAnti-DAGK6 antibody |
Information sourced from Uniprot.org