• Western blot analysis of lysates from K562 cells, using DGKE Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-DGKE antibody (161-210 aa) (STJ92699)

SKU:
STJ92699

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Diacylglycerol kinase epsilon (161-210 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DGKE
Gene ID: 8526
Uniprot ID: DGKE_HUMAN
Immunogen Region: 161-210 aa
Specificity: DGK-Epsilon Polyclonal Antibody detects endogenous levels of DGK-Epsilon protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human DGKE at the amino acid range 161-210
Function Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently.
Protein Name Diacylglycerol Kinase Epsilon
Dag Kinase Epsilon
Diglyceride Kinase Epsilon
Dgk-Epsilon
Database Links Reactome: R-HSA-114508
Cellular Localisation Membrane
Single-Pass Membrane Protein
Cytoplasm
Alternative Antibody Names Anti-Diacylglycerol Kinase Epsilon antibody
Anti-Dag Kinase Epsilon antibody
Anti-Diglyceride Kinase Epsilon antibody
Anti-Dgk-Epsilon antibody
Anti-DGKE antibody
Anti-DAGK5 antibody

Information sourced from Uniprot.org

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