• STJ71070 (0. 03µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71070 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71070 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-DGAT1 antibody (Internal) (STJ71070)

SKU:
STJ71070-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DGAT1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.01-0.1.0µg/ml
IHC-3.75µg/ml
IF-Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DGAT1
Gene ID: 8694
Uniprot ID: DGAT1_HUMAN
Immunogen Region: Internal
Accession Number: NP_036211.2
Immunogen Sequence: QNSMKPFKDMDYS
Protein Name Diacylglycerol O-Acyltransferase 1
Acat-Related Gene Product 1
Acyl-Coa Retinol O-Fatty-Acyltransferase
Arat
Retinol O-Fatty-Acyltransferase
Diglyceride Acyltransferase
Database Links Reactome: R-HSA-1482883
Reactome: R-HSA-6798695
Reactome: R-HSA-75109
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Diacylglycerol O-Acyltransferase 1 antibody
Anti-Acat-Related Gene Product 1 antibody
Anti-Acyl-Coa Retinol O-Fatty-Acyltransferase antibody
Anti-Arat antibody
Anti-Retinol O-Fatty-Acyltransferase antibody
Anti-Diglyceride Acyltransferase antibody
Anti-DGAT1 antibody
Anti-AGRP1 antibody
Anti-DGAT antibody

Information sourced from Uniprot.org

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