• STJ71803 (0. 5µg/ml) staining of Mouse Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71803 (8µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ71803 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody

Anti-Desmin antibody (C-Term) (STJ71803)

SKU:
STJ71803-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Desmin (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-8µg/ml
ELISA-antibody detection limit dilution 1:2000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DES
Gene ID: 1674
Uniprot ID: DESM_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001918.3
Immunogen Sequence: RDGEVVSEATQQQHE
Function Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin.
Protein Name Desmin
Database Links Reactome: R-HSA-390522
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
Z Line
Cell Membrane
Sarcolemma
Nucleus
Localizes In The Intercalated Disks Which Occur At The Z Line Of Cardiomyocytes
Localizes In The Nucleus Exclusively In Differentiating Cardiac Progenitor Cells And Premature Cardiomyocytes
Alternative Antibody Names Anti-Desmin antibody
Anti-DES antibody

Information sourced from Uniprot.org

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