Anti-DES antibody (400-470 aa) [ABT168] (STJ197254)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2bk
ImmunogenSynthesized peptide derived from the human Desmin at the amino acid range 400-470
General Information
| Short Description | Mouse monoclonal anti-Desmin (400-470 aa) for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ABT168 |
| Isotype | IgG2bk |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | IHC-P 1:200-400WB 1:200-1000 |
| Formulation | Liquid in PBS pH7.2, 0.03% Proclin 300, with stabilizing protein. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DES |
| Gene ID | 1674 |
| Uniprot ID | DESM_HUMAN |
| Immunogen | Synthesized peptide derived from the human Desmin at the amino acid range 400-470 |
| Immunogen Region | 400-470 aa |
| Specificity | The antibody can specifically recognize human Desmin protein. |
Additional Info
| Function | Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin. |
| Protein Name | Desmin |
| Database Links | Reactome: R-HSA-390522 |
| Cellular Localisation | CytoplasmMyofibrilSarcomereZ LineCell MembraneSarcolemmaNucleusCell TipNucleus EnvelopeLocalizes In The Intercalated Disks Which Occur At The Z Line Of CardiomyocytesLocalizes In The Nucleus Exclusively In Differentiating Cardiac Progenitor Cells And Premature CardiomyocytesPkp2 Is Required For Correct Anchoring Of Des At The Cell Tip And Nuclear Envelope |
| Alternative Antibody Names | Anti-Desmin antibodyAnti-DES antibody |
Information sourced from Uniprot.org