• Western blot analysis of lysates from U-87MG cells, using MLD Rabbit monoclonal antibody (STJ11104192) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DEGS1 antibody (1-100) [S4192RM] (STJ11104192)

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STJ11104192

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Sphingolipid Delta (4-Desaturase Des1 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4192RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DEGS1
Gene ID: 8560
Uniprot ID: DEGS1_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MLD (NP_003667.1).
Immunogen Sequence: MGSRVSREDFEWVYTDQPHA DRRREILAKYPEIKSLMKPD PNLIWIIIMMVLTQLGAFYI VKDLDWKWVIFGAYAFGSCI NHSMTLAIHEIAHNAAFGNC
Tissue Specificity Ubiquitous.
Post Translational Modifications Myristoylation can target the enzyme to the mitochondria leading to an increase in ceramide levels.
Function Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). Catalyzes the equilibrium isomerization of retinols.
Protein Name Sphingolipid Delta(4-Desaturase Des1
Cell Migration-Inducing Gene 15 Protein
Degenerative Spermatocyte Homolog 1
Dihydroceramide Desaturase-1
Membrane Lipid Desaturase
Retinol Isomerase
Database Links Reactome: R-HSA-1660661
Reactome: R-HSA-6798695
Cellular Localisation Mitochondrion Membrane
Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Sphingolipid Delta(4-Desaturase Des1 antibody
Anti-Cell Migration-Inducing Gene 15 Protein antibody
Anti-Degenerative Spermatocyte Homolog 1 antibody
Anti-Dihydroceramide Desaturase-1 antibody
Anti-Membrane Lipid Desaturase antibody
Anti-Retinol Isomerase antibody
Anti-DEGS1 antibody
Anti-DES1 antibody
Anti-MLD antibody
Anti-MIG15 antibody

Information sourced from Uniprot.org

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