Anti-DEFB4A antibody (1-64 aa) (STJ11107083)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DEFB4A (1-64 aa) antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DEFB4A.DEFB4B |
| Gene ID | 100289462/1673 |
| Uniprot ID | DFB4A_HUMAN |
| Immunogen Region | 1-64 aa |
| Immunogen Sequence | MRVLYLLFSFLFIFLMPLPG VFGGIGDPVTCLKSGAICHP VFCPRRYKQIGTCGLPGTKC CKKP |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-64 of human DEFB4A (NP_004933.1). |
Additional Info
| Tissue Specificity | Expressed in lung epithelial cells (at protein level). Expressed in foreskin, lung and trachea. Lower expression in kidney, uterus and salivary gland tissue. Expressed in epithelial cells of the respiratory tract, with higher expression in distal parenchyma of the lung, trachea, and tonsils, and lower expression in pharynx and adenoid, and low expression in tongue and larynx. |
| Function | Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria. Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM. Also exhibits antimicrobial activity against the yeast C.albicans. Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death. Acts as a ligand for C-C chemokine receptor CCR6. Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells. |
| Protein Name | Defensin Beta 4aBeta-Defensin 2Bd-2Hbd-2Defensin - Beta 2Skin-Antimicrobial Peptide 1Sap1 |
| Database Links | Reactome: R-HSA-1461957Reactome: R-HSA-1461973 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Defensin Beta 4a antibodyAnti-Beta-Defensin 2 antibodyAnti-Bd-2 antibodyAnti-Hbd-2 antibodyAnti-Defensin - Beta 2 antibodyAnti-Skin-Antimicrobial Peptide 1 antibodyAnti-Sap1 antibodyAnti-DEFB4A antibodyAnti-DEFB102 antibodyAnti-DEFB2 antibodyAnti-DEFB4.DEFB4B antibody |
Information sourced from Uniprot.org