• Western blot analysis of extracts of various cell lines, using DDX6 antibody (STJ118705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using DDX6 antibody (STJ118705) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human mammary cancer using DDX6 antibody (STJ118705) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using DDX6 antibody (STJ118705) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using DDX6 Polyclonal Antibody (STJ118705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DDX6 antibody (414-533) (STJ118705)

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STJ118705

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDX6 (414-533) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX6
Gene ID: 1656
Uniprot ID: DDX6_HUMAN
Immunogen Region: 414-533
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 414-533 of human DDX6 (NP_001073280.1).
Immunogen Sequence: DYEARPQYYKLIEECISQIV LHKNGADPDFKCRHLQIEIE GLIDQMIDKTKVEKSEAKAA ELEKKLDSELTARHELQVEM KKMESDFEQKLQDLQGEKDA LHSEKQQIATEKQDLEAEVS
Tissue Specificity Abundantly expressed in most tissues.
Post Translational Modifications Sumoylated.
Function Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
Protein Name Probable Atp-Dependent Rna Helicase Ddx6
Atp-Dependent Rna Helicase P54
Dead Box Protein 6
Oncogene Rck
Database Links Reactome: R-HSA-430039
Cellular Localisation Cytoplasm
P-Body
Nucleus
Imported In The Nucleus Via Interaction With Eif4enif1/4e-T Via A Piggy-Back Mechanism
Upon Cellular Stress
Relocalizes To Stress Granules
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx6 antibody
Anti-Atp-Dependent Rna Helicase P54 antibody
Anti-Dead Box Protein 6 antibody
Anti-Oncogene Rck antibody
Anti-DDX6 antibody
Anti-HLR2 antibody
Anti-RCK antibody

Information sourced from Uniprot.org

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