• Western blot analysis of extracts of various cell lines, using DDX6 rabbit monoclonal antibody (STJ11102512) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH-3T3 cells using DDX6 Rabbit monoclonal antibody (STJ11102512) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using DDX6 rabbit monoclonal antibody (STJ11102512) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using DDX6 Rabbit monoclonal antibody (STJ11102512) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using DDX6 rabbit monoclonal antibody (STJ11102512) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DDX6 Rabbit monoclonal antibody (STJ11102512) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDX6 antibody (101-200) [S2MR] (STJ11102512)

SKU:
STJ11102512

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DDX6 (101-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX6
Gene ID: 1656
Uniprot ID: DDX6_HUMAN
Immunogen Region: 101-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 101-200 of human DDX6 (P26196).
Immunogen Sequence: YCLKRELLMGIFEMGWEKPS PIQEESIPIALSGRDILARA KNGTGKSGAYLIPLLERLDL KKDNIQAMVIVPTRELALQV SQICIQVSKHMGGAKVMATT
Tissue Specificity Abundantly expressed in most tissues.
Post Translational Modifications Sumoylated.
Function Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
Protein Name Probable Atp-Dependent Rna Helicase Ddx6
Atp-Dependent Rna Helicase P54
Dead Box Protein 6
Oncogene Rck
Database Links Reactome: R-HSA-430039
Cellular Localisation Cytoplasm
P-Body
Nucleus
Imported In The Nucleus Via Interaction With Eif4enif1/4e-T Via A Piggy-Back Mechanism
Upon Cellular Stress
Relocalizes To Stress Granules
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx6 antibody
Anti-Atp-Dependent Rna Helicase P54 antibody
Anti-Dead Box Protein 6 antibody
Anti-Oncogene Rck antibody
Anti-DDX6 antibody
Anti-HLR2 antibody
Anti-RCK antibody

Information sourced from Uniprot.org

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