• Western blot analysis of extracts of various cell lines, using DDX47 antibody (STJ112396) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-DDX47 antibody (186-455) (STJ112396)

SKU:
STJ112396

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDX47 (186-455) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX47
Gene ID: 51202
Uniprot ID: DDX47_HUMAN
Immunogen Region: 186-455
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human DDX47 (NP_057439.2).
Immunogen Sequence: TEVDKILKVIPRDRKTFLFS ATMTKKVQKLQRAALKNPVK CAVSSKYQTVEKLQQYYIFI PSKFKDTYLVYILNELAGNS FMIFCSTCNNTQRTALLLRN LGFTAIPLHGQMSQSKRLGS LNKFKAKARSILLATDVASR GLDIPHVDVVVNFDIPTHSK DYIHRVGRTARAGRSGKAIT FVTQYDVELFQRIEHLIGKK LPGFPTQDDEVMMLTERVAE AQRFARMELREHGEKKKRS
Tissue Specificity Expressed in skin, lung and breast. Also expressed in the brain.
Function Required for efficient ribosome biogenesis. May have a role in mRNA splicing. Involved in apoptosis.
Protein Name Probable Atp-Dependent Rna Helicase Ddx47
Dead Box Protein 47
Database Links Reactome: R-HSA-6790901
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Localizes In The Nucleolar-Organizing Region During Ribosome Biogenesis
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx47 antibody
Anti-Dead Box Protein 47 antibody
Anti-DDX47 antibody

Information sourced from Uniprot.org

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