• Immunohistochemistry analysis of DDX46 in paraffin-embedded mouse testis using DDX46 Rabbit polyclonal antibody (STJ117810) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DDX46 in paraffin-embedded rat testis using DDX46 Rabbit polyclonal antibody (STJ117810) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using DDX46 Rabbit polyclonal antibody (STJ117810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DDX46 antibody (682-1031) (STJ117810)

SKU:
STJ117810

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDX46 (682-1031) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX46
Gene ID: 9879
Uniprot ID: DDX46_HUMAN
Immunogen Region: 682-1031
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 682-1031 of human DDX46 (NP_055644.2).
Immunogen Sequence: GLDVKHLILVVNYSCPNHYE DYVHRAGRTGRAGNKGYAYT FITEDQARYAGDIIKALELS GTAVPPDLEKLWSDFKDQQK AEGKIIKKSSGFSGKGFKFD ETEQALANERKKLQKAALGL QDSDDEDAAVDIDEQIESMF NSKKRVKDMAAPGTSSVPAP TAGNAEKLEIAKRLALRINA QKNLGIESQDVMQQATNAIL RGGTILAPTVSAKTIAEQLA EKINAKLNYVPLEKQEEER
Function Plays an essential role in splicing, either prior to, or during splicing A complex formation.
Protein Name Probable Atp-Dependent Rna Helicase Ddx46
Dead Box Protein 46
Prp5 Homolog
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus Speckle
Nucleus
Cajal Body
Membrane
Lipid-Anchor
Present In Cajal Bodies (Cbs) And Nuclear Speckles
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx46 antibody
Anti-Dead Box Protein 46 antibody
Anti-Prp5 Homolog antibody
Anti-DDX46 antibody
Anti-KIAA0801 antibody

Information sourced from Uniprot.org

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