• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g DDX41 antibody (STJ28659). Western blot was performed from the immunoprecipitate using DDX41 antibody (STJ28659) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using DDX41 Rabbit polyclonal antibody (STJ28659). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DDX41 in paraffin-embedded mouse testis using DDX41 Rabbit polyclonal antibody (STJ28659) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DDX41 in paraffin-embedded rat testis using DDX41 Rabbit polyclonal antibody (STJ28659) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DDX41 in paraffin-embedded rat kidney using DDX41 Rabbit polyclonal antibody (STJ28659) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat testis, using DDX41 Rabbit polyclonal antibody (STJ28659) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DDX41 antibody (1-300) (STJ28659)

SKU:
STJ28659

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDX41 (1-300) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX41
Gene ID: 51428
Uniprot ID: DDX41_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).
Immunogen Sequence: MEESEPERKRARTDEVPAGG SRSEAEDEDDEDYVPYVPLR QRRQLLLQKLLQRRRKGAAE EEQQDSGSEPRGDEDDIPLG PQSNVSLLDQHQHLKEKAEA RKESAKEKQLKEEEKILESV AEGRALMSVKEMAKGITYDD PIKTSWTPPRYVLSMSEERH ERVRKKYHILVEGDGIPPPI KSFKEMKFPAAILRGLKKKG IHHPTPIQIQGIPTILSGRD MIGIAFTGSGKTLVFTLPV
Function Probable ATP-dependent RNA helicase. Is required during post-transcriptional gene expression. May be involved in pre-mRNA splicing.
Protein Name Probable Atp-Dependent Rna Helicase Ddx41
Dead Box Protein 41
Dead Box Protein Abstrakt Homolog
Database Links Reactome: R-HSA-1834941
Reactome: R-HSA-3134975
Reactome: R-HSA-3270619
Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Probable Atp-Dependent Rna Helicase Ddx41 antibody
Anti-Dead Box Protein 41 antibody
Anti-Dead Box Protein Abstrakt Homolog antibody
Anti-DDX41 antibody
Anti-ABS antibody

Information sourced from Uniprot.org

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